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. 2021 Jan 14;11:1347. doi: 10.1038/s41598-020-79971-y

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Establishment of iron deficiency anemia model in female rats. (a) Scheme of IDA model establishment and iron supplement treatment. The whole blood of maternal rats was collected and haematological indexes were tested. (b) HGB. (c) HCT. (d) MCV. (e) CHCM. f CH. (g) RDW. The serum of maternal rats was collected and tested. (h) SI. (i) SF. (j) TS. (k) TIBC. HGB haemoglobin, HCT Hematocrit, MCV mean corpuscular volume, CHCM cell haemoglobin concentration mean, CH haemoglobin content of red blood cell, RDW red cell volume distribution width, SI serum iron, SF serum ferritin, TS transferrin saturation, TIBC total iron binding capacity. Data are presented as mean ± SD (n = 12). **p < 0.01, compared with NG. Unpaired t-test was used for comparison between two groups.