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. 2021 Jan 14;11:1347. doi: 10.1038/s41598-020-79971-y

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Iron related indexes of maternal rats after iron supplement treatment. The serum of maternal rats was collected and tested. (a) SI. (b) SF. (c) TS. (d) TIBC. Data are presented as mean ± SD (n = 6). Western blot analysis for FTL and Tf in liver (e–g) and spleen (h–j). The quantification of western blotting was provided in supplementary material. Data of Western blot analysis (mean ± SD) are expressed as the ratio of the relative contents between the value from IDA group and NG group and six iron treatment groups (n = 3). The relative contents of target proteins were quantified using the ratio between the optical density (OD) of target protein and the amount of the housekeeping protein GAPDH. SI serum iron, SF serum ferritin, TS transferrin saturation, TIBC total iron binding capacity, FTL ferritin light chain, Tf transferrin. *p < 0.05, **p < 0.01, compared with NG, ^#p < 0.05, ^##p < 0.01, compared with IDAG. One-way ANOVA followed by Tukey multiple comparison test was used for comparison among 8 different groups.