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. 2021 Jan 14;11:1393. doi: 10.1038/s41598-021-81164-0

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Skipped exon event in PGAP2. (a) Sashimi plots for PGAP2 inclusion/exclusion of exon 4 in the LNCaP and VCaP models. To analyze the expression of the included and excluded forms, LNCaP and LNCaP-AR-V7 cells were transferred to CSS medium and treated with vehicle, R1881, or Dox for 24 h, and RNA was isolated. (b) qPCR analysis of the region encompassing Exon 4 and 5 (long isoform) in the LNCaP model showed the expected increase in expression. Expression values were plotted as a mean of three biological replicates from the same experiment. Error bars represent SEM. Asterisks (*) signify values that differed significantly with respect to vehicle (P < 0.05 in Student's t-test). (c) Agarose gel analyses of products amplified using one primer in exon 3 and one in exon 5. The bottom band of 275 bp represents the excluded form; the top band 458 bp represents long (Exon 4 included) form. There is an additional intermediate band suggesting that there is an additional sequence, which may represent an unknown exon.