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. 2021 Jan 14;11:1393. doi: 10.1038/s41598-021-81164-0

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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A novel exon exists between exons 3 and 4. (a) IGV plot showing the presence of Novel Exon (NE) reads in PGAP2 in the LNCaP cell line and locations of sequence overlap containing a portion of the novel exon. (b) IGV peak reads of PGAP2 in the VCaP model also shows a transcribed region between exons 3 and 4. (c) Semi-quantitative PCR spanning Novel Exon (NE) and exon 5. The 295 bp band is the excluded form and the top band (478 bp) is the long (Exon 4 included) form. The middle band may correspond to the novel exon linked to another skipped exon between exons 4 and 5 linked to exon 5. (d, e) qPCR validation of novel exon linked to Exon 4 in LNCaP and VCaP models respectively.