Figure 5.

Both AR isoforms bind to the region containing the novel exon. (a) shows the ChIP-exo tracks showing binding of AR and AR-V7 to the novel exon region and (b) shows ChIP-qPCR validation of AR and AR-V7 binding in LNCaP AR-V7 cells. LNCaP AR-V7 cells were treated with Vehicle, R1881, or Dox and after 24 h ChIP was performed with an amino-terminal AR antibody, and rabbit IgG was used as a negative IP control. Quantitative PCR was performed with primers specific for the novel exon of the PGAP2 gene. It shows the recovery expressed as the relative amount of immunoprecipitated DNA normalized to input DNA after qPCR analysis. Expression values were plotted as a mean of two biological replicates from the same experiment. Error bars represent SEM. Asterisks (*) signify values that differed significantly with respect to immunoprecipitated vehicle (P < 0.05 in Student's t-test). Each experiment was performed a minimum of three times and a representative experiment was shown.