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. 2021 Jan 14;11:1393. doi: 10.1038/s41598-021-81164-0

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Alternative first exon event in TPD52. (a) IGV RNA expression reads of both isoforms of TPD52 in LNCaP Vehicle, LNCaP R1881, LNCaP-AR-V7 Dox treated samples, VCaP Vehicle, VCaP R1881, and VCaP-AR-V7 Dox treated samples, ChIP-exo tracks in LNCaP R1881 and LNCaP-AR-V7 Dox treated samples and a FOXA1 ChIP track in the LNCaP cell line. (b) To analyze the expression of the promoter 2 and the promoter 1 isoforms, LNCaP and LNCaP-AR-V7 cells were transferred to CSS medium and treated with vehicle, R1881, or Dox for 24 h and RNA was isolated and subjected to qPCR. (c) LNCaP-AR-V7 cells were treated with control si or FOXA1-siRNA and cells were harvested at 72 h for western blotting and probed with FOXA1 and tubulin antibodies. (d) LNCaP-AR-V7 cells were treated with control si or si-FOXA1 for 48 h followed by treatment with vehicle, R1881, or Dox for an additional 24 h. RNA was isolated subjected to qPCR with primers for promoter 2 and promoter 1 isoforms.