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The behavior of the longitudinal magnetization (Mz) was simulated by means of Bloch equation simulations, by assuming the labeled blood (in labeling image) and non- labeled blood (in control image) enter the imaging volume and receive excitation RF-pulses over 1400 ms. The transverse magnetization was assumed to be spoiled before the next excitation pulse is applied. The T1 and T2 of blood were assumed to be 1650 ms9 and 150 ms,78 respectively. RF, radiofrequency; FA, flip-angle.
