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. 2021 Jan 14;11:1404. doi: 10.1038/s41598-020-79115-2

Table 3.

Comparison of average physiological indices regarding ABA dose response for germination inhibition of Ler and Col-0 accessions.

Ecotype ABA conc. Gmax (std) t20 (std) t50 (std) t80 (std) U80-20 (std) Nb. of wells
Col-0 0 μm 96.56 (8.3) 29.58 (2.15) 33.42 (1.62) 36.25 (1.71) 6.67 (2.35) 12
1 μm 64.19 (30.4) 39.25 (7.70) 52.33 (14.58) 65.17 (16.83) 25.92 (15.01) 12
3 μm 31 (18.53) 45.91 (23.89) 67 (26.74) 88.91 (18.91) 43 (23.96) 12
10 μm 3.88 (6.53) 79.25 (32.39) 81.75 (33.09) 84.5 (6.5) 5.25 (3.54) 12
Ler 0 μm 80.91 (16.06) 36.75 (5.31) 43.92 (3.58) 53.50 (6.5) 18.75 (6.05) 12
1 μm 46.02 (24.26) 50.17 (13.22) 61.08 (12.31) 78.42 (17.24) 28.25 (22.82) 12
3 μm 8.60 (8.84) 61 (12.84) 68.33 (16.81) 72.78 (20.80) 11.78 (17.58) 12
10 μm 3.02 (4.16) 73.25 (15.99) 73.25 (15.99) 73.25 (15.99) NA 12

In brackets are the standard deviations of the means. In stressed condition, only wells with more than 2 germinated seeds were used to compute the averages of t20, t50, t80 and U80-20 respectively. Treatments have been randomized (c.f. Supplementary Data S5 online). The experiment has been done from a single microplate in order to illustrate that expected effect of ABA can be detected from a single microplate. If one would like to draw conclusions regarding the impact of ABA on germination, multiple independent analyses would be required.