Figure 1.

AAV2 capsid library design

(A) AAV2 amino acid residue sequence for VP1 where variable residue mutations (33 residues) and constant residue mutations (9 residues) are highlighted. (B) Three-dimensional model of a VP1 monomeric protein highlighting mutations to the backbone for the DGE-DF motif, as well as the antibody and proteasome evading mutations, S384T, Q385N, Y444F, and S498T. (C) Assembled AAV2 capsid with 60 monomers, highlighting the positions for the DGE-DF motif and antibody evading mutations made to the backbone of the library. (D) VP1 monomeric protein highlighting mutations for the 33 residues within the VRs that will receive variable mutations. (E) Assembled AAV2 capsid with 60 monomers, highlighting the mutated residues within the VRs. (F) Table specifying the 33 codons and potential residue outcomes for each variable residue receiving mutations in this study.