Figure 2.

Library characterization

(A) Series of Venn diagrams depicting the sequence distribution of parental plasmid and viral progeny libraries. Left is a Venn diagram showing the sequencing results after NGS, illustrating how many of the viral progeny variants were not sequenced in the parental plasmid library. Center is a Venn diagram showing the estimated distribution of reads for the parental plasmid and viral progeny libraries, with the assumption that the majority of the viral progeny sequences were sequenced. Right is a Venn diagram representing how a more rational design of libraries would result in an increase in variants expected to make it to the viral progeny library. (B) Sequence logos illustrating the composition of residues of libraries derived from NGS data. Top is the probability distribution for positions from NGS of the parental plasmid library. Bottom is the probability distribution for positions from NGS of the viral progeny library.