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. 2021 Jan 14;11:1312. doi: 10.1038/s41598-020-79218-w

Figure 2.

Figure 2

Functional characterization of GBR12909 on DAT in U2OS-DAT cells in a TRACT assay. Cells were pretreated with vehicle, 10 µM (a,b) or increasing concentrations (c,d) of GBR12909. Representative vehicle-corrected xCELLigence traces after stimulation with (a) increasing concentrations of dopamine or (c) 3.16 µM dopamine. (b) Concentration-effect curves of dopamine in U2OS-DAT cells pretreated with vehicle or 10 µM GBR12909 are shown as the net AUC of the first 120 min after stimulation normalized to the cell response of 31.6 µM dopamine. (d) Concentration-effect curve of GBR12909 after addition of 3.16 µM dopamine normalized to 10 µM GBR12909. Data are shown as mean ± SEM of three to six separate experiments each performed in duplicate.