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. 2021 Jan 14;11:1312. doi: 10.1038/s41598-020-79218-w

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© The Author(s) 2021, corrected publication 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Representative saturation binding curve of [³H]WIN35,428 to DAT on U2OS-DAT membranes. Total binding (●) and non-specific binding (○) were determined in the absence or presence of 100 μM GBR12909. Specific binding (■) was obtained by linear subtraction of non-specific binding from total binding. Data are shown as the mean of a representative graph of three separate experiments each performed in triplicate.