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. 2021 Jan 14;11:1312. doi: 10.1038/s41598-020-79218-w

Figure 5.

Figure 5

Functional characterization of GBR12909 and cocaine on DAT in JumpIn-DAT cells in the presence of 1 µg/ml dox (+dox) in a TRACT assay. Cells were pretreated with vehicle or 10 µM (a,b) or increasing concentrations (c,d) of either GBR12909 or cocaine. (a) Representative vehicle-corrected xCELLigence traces after stimulation with increasing concentrations of dopamine or (c) 3.16 µM dopamine. (b) Concentration-effect curves of dopamine in dox-treated JumpIn-DAT cells pretreated with vehicle or 10 µM GBR12909 or cocaine are shown as the net AUC of the first 30 min after stimulation normalized to the cell response of 316 µM dopamine. (d) Concentration-effect curve of GBR12909 and cocaine after addition of 10 µM dopamine normalized to 10 µM inhibitor. Data are shown as mean ± SEM of four to nine separate experiments each performed in duplicate.