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. 2021 Jan 14;11:1399. doi: 10.1038/s41598-021-80999-x

Figure 1.

Figure 1

SHP2 inhibition in cancer cells enhances immune cells-mediated tumor killing. (a) Absolute tumor cell counts from each well of 384-well Elplasia plate after 6 days co-culture of OVCAR-8 spheroids with human PBMCs (2 donors). Relative percentage of absolute tumor counts (SHP099 treated over DMSO group) is labeled. (b) Absolute tumor cell counts of paired DMSO and SHP099 (20 μM) treated co-culture groups of OVCAR-8 spheroids with PBMCs from multiple replicates with different donors. (c) Absolute tumor cell counts of paired DMSO and SHP099 (20 μM) treated tumor only groups from multiple replicates. (d) Absolute tumor cell counts from each well of 384-well Elplasia plate after 6 days co-culture of OVCAR-8 spheroids with human PBMCs (3 donors). Relative percentage of absolute tumor counts (TNO155 treated over DMSO group) is labeled. (e) Absolute tumor cell counts of paired DMSO and TNO155 (1 μM) treated co-culture groups of OVCAR-8 spheroids with PBMCs from multiple replicates with different donors. (f) Absolute tumor cell counts of paired DMSO and TNO155 (1 μM) treated tumor only groups from multiple replicates. (g) Absolute tumor cell counts from each well of 384-well Elplasia plate after 6 days co-culture of OVCAR-8-CAS9-sgPTPN11-1 or OVCAR-8-CAS9-sgPTPN11-2 spheroids with human PBMCs (3 donors). OVCAR-8 cells were treated with or without doxycycline (100 ng/ml) for 5 days before co-culture. Relative percentage of absolute tumor counts (Dox+ over Dox− group) is labeled. (h) Absolute tumor cell counts from each well of 384-well Elplasia plate after 6 days co-culture of OVCAR-8-SHP2-WT or OVCAR-8-SHP2-T253M/Q257L spheroids with human PBMCs. OVCAR-8 cells were treated with or without doxycycline (100 ng/ml) for 5 days before co-culture. Relative percentage of absolute tumor counts (SHP099 treated over DMSO group) is labeled.