Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Jan 14;11:1399. doi: 10.1038/s41598-021-80999-x

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

SHP2 inhibition upregulates expression of CXCR3 ligands and promotes immune cell infiltration. (a) Heatmap of transcriptional expression level of CXCR3 ligand genes CXCL9, CXCL10, CXCL11 in OVCAR-8 tumor cells in co-culture from scRNAseq data. (b) Volcano plot of transcriptional expression level of CXCR3 ligand genes CXCL9, CXCL10, CXCL11 in OVCAR-8 tumor cells in co-culture from scRNAseq data. (c) Luminex analysis of TNO155 and SHP099-induced fold change of a panel of cytokines (normalized to control group) in supernatants collected from co-culture of OVCAR-8 spheroids with PBMCs. (d) Flow cytometry analysis of intracellular staining of CXCL10 from 2 days of co-culture and tumor only. CXCL10-positive cells are gated. Percentage of CXCL10-positive population is labeled. (e) Light sheet microscopy imaging of infiltrated PBMCs (Green) inside OVCAR-8 tumor spheroids (Red) after 24 h of co-culture. Scale bar: 50 μm. (f, h) Histogram of number of total infiltrated PBMCs inside tumor spheroids. (g, i) Histogram of number of infiltrated PBMCs in different layers of tumor spheroids. Flow cytometry data was analyzed and processed with FlowJo (Version 10.7.1, https://www.flowjo.com/solutions/flowjo/downloads/previous-versions).