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. 2021 Jan 14;11:1423. doi: 10.1038/s41598-020-79858-y

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Experimentally measured electroporated area (dark area) versus pulse number for an EP-based protocol (4, 8, 16 and 32 pulses of $100 μ s$ , $1 Hz$ with an amplitude of $1500 V$ ), and superposed, the predicted threshold variation versus pulse number obtained with the OpenEP simulator (Figure taken from Marino et al.¹⁰).