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. 2021 Jan 13;30:0963689720975390. doi: 10.1177/0963689720975390

Figure 1.

Figure 1.

Hsa_circ_0023409 was upregulated in GC tissues and cells and associated with the prognosis of GC patients. (A) The mRNA expression levels of hsa_circ_0023409 in 87 paired GC tissues and adjacent normal tissues. (B) In situ hybridization analysis of hsa_circ_0023409 with locked nuclei acid probes in GC tissues and adjacent tissues. Scale bar =200 μm (100x) and 100 μm (200x). (C) Overall survival rate of GC patients with high hsa_circ_0023409 expression or low hsa_circ_0023409 expression. (D) The mRNA expression levels of hsa_circ_0023409 in GC cells (AGS, MKN45, HGC-27, SUN-1, and MKN7) and normal gastric cells (GES-1). (E) Relative mRNA expression levels of hsa_circ_0023409 and its linear RNA RNF 121 in HGC-27 and MKN45 cells after RNase R treatment. (F) Relative expression of hsa_circ_0023409 and glyceraldehyde 3-phosphate dehydrogenase in nucleus and cytoplasm in HGC-27 and MKN45 cells. Values are the mean ± standard deviation. **P < 0.01. GC: gastric cancer; mRNA: micro RNA.