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. 2021 Jan 13;30:0963689720975390. doi: 10.1177/0963689720975390

Figure 5.

Figure 5.

Hsa_circ_0023409 activated IRS4/PI3K/AKT pathway via sponging miR-542-3p. (A) Binding sites of miR-542-3p to IRS4 predicted by TargetScanHuman 7.2. (B) The luciferase activity in HGC-27 and MKN45 cells transfected with miR-542-3p mimics or inhibitor. (C) The protein levels of IRS4, Akt, and p-Akt in MKN45 cells transfected with miR-542-3p inhibitor and in HGC-27 cells transfected with miR-542-3p mimics. (D) The mRNA expression levels of IRS4 in 87 paired GC tissues and adjacent normal tissues. (E) Correlation analysis of IRS4 with hsa_circ_0023409 and miR-542-3p in GC. (F) The protein levels of IRS4, Akt, and p-Akt in hsa_circ_0023409 downregulated cells treated with miR-542-3p mimics or inhibitor. Values are the mean ± standard deviation. **P < 0.01 and ## P < 0.01. circRNA: circular RNA; GC: gastric cancer; IRS4: insulin receptor substrate 4; NC: negative control; PI3K: phosphatidylinositol 3-kinase; UTR: untranslated region; WT: wild type.