Figure 6.

Reduced intestinal lipid absorption in NT-PGC-1α^−/− female mice fed HFD. (A) Uptake of [¹⁴C]-palmitic acids in jejunal enterocytes isolated from female WT and NT-PGC-1α^−/− mice fed HFD (n = 5 per group). Data are presented as the mean ± SEM. **P < 0.01 determined by Student’s t test. (B) Quantitative real time PCR analysis of genes involved in intestinal fat absorption and secretion in the jejunum of female WT and NT-PGC-1α^−/− mice fed HFD (n = 6–7 per group). Data are presented as the mean ± SEM. *P < 0.05 determined by Student’s t test. (C) Oral fat tolerance test. Female WT and NT-PGC-1α^−/− mice fed HFD for 8 weeks were fasted overnight and administered by gavage of olive oil. Blood samples were collected at indicated times and analyzed for serum TG concentrations (n = 7 per group). Two-way ANOVA was used to determine the differences in serum TG levels between the genotypes. F (1, 60) = 7.941, **P = 0.0065.