Fig. 1. Generation and characterisation of ETV2-ECs.

A H9 human embryonic stem cells engineered with an RMCE cassette were recombined with a doxycycline-inducible ETV2 overexpression cassette (method described in and figure adapted from Ordovas et al.⁴¹). (FRT Flippase recognition target, SA splice acceptor, PuroR puromycin resistance gene, TRE tetracycline response element, M2rtTA M2 reverse tetracycline transactivator.) B Schematic representation for PSC differentiation into endothelial cells. C Gene expression (RT-qPCR) profile of ETV2, the endothelial markers CDH5, PECAM1, and VEGFR2, and the endothelial TFs FLI1 and ERG. Samples were collected every 2–4 days of differentiation until day 16 with N = 3 biological replicates per time point. Expression is relative to that of day 0 of differentiation (i.e., stem cells). D Confocal image of phalloidin-stained ETV2-ECs in Matrigel showing tube formation.