Figure 2.

Progranulin depletion decreases the phosphorylation of proteins in the Akt/mTOR pathway. a) Kasumi-1 cells were cultured with anti progranulin antibody (200 μg/ml) or control antibody for the indicated times. Whole cell lysate was collected and expression level of protein was analyzed by western blotting. b) Phosphorylation level of protein was normalized to that of each corresponding total protein. Error bars indicate the SD from mean; n = 3. (∗∗P < 0.01, ∗∗∗P < 0.001; two-tailed Student's t test) c) Progranulin specific siRNA or control siRNA transfected Kasumi-1 cells were cultured with rapamycin (20 nM). Proliferation of treated cells was determined by MTT assay. Error bars indicate the SD from mean; n = 3. (∗∗∗P < 0.001 between Ctrl and PGRN KD, †††P < 0.001 between Ctrl + rapamycin and PGRN KD + rapamycin, ‡‡‡P < 0.001 between PGRN KD and PGRN KD + rapamycin; Tukey's test).