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. 2021 Jan 8;7(1):e05849. doi: 10.1016/j.heliyon.2020.e05849

Figure 5.

Figure 5

Blocking of the TGF-β signaling axis attenuates inhibition of cell proliferation by progranulin depletion. a) Kasumi-1 cells transfected progranulin-specific siRNA or control siRNA were cultured with or without anti TGF-β antibody (2 μg/ml) for 48 h. Whole cell lysate was collected and expression of cleaved PARP was analyzed by Western blotting. b) Expression level of cleaved PARP was normalized to that of β-actin. Error bars indicate the SD from mean; n = 4. (∗∗P < 0.01 compared with ctrl. ††P < 0.01, †††P < 0.001 compared with PGRN KD; Tukey's test) c) Progranulin specific siRNA or control siRNA transfected Kasumi-1 cells were cultured with the combination of LY2109761 (250 μM). d) or anti TGF-β antibody (2 μg/ml). Proliferation of treated cells was determined by MTT assay. Error bars indicate the SD from mean; n = 3. (∗∗∗P < 0.001 between Ctrl and PGRN KD. †P < 0.05, ††P < 0.01 between PGRN KD and PGRN KD + LY2109761. ‡P < 0.05, ‡‡‡P < 0.001 between PGRN KD and PGRN KD + TGF-β Ab; Tukey's test).