Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Jan 14;11:1433. doi: 10.1038/s41598-021-81081-2

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Super-resolution reconstructions and cellular distributions of SecA-Ypet under different conditions. (A,B) SecA under native conditions, (C,D) SecA-mediated translocation is blocked by 3 mM NaN₃ (E,F) protein translocation is impaired due to collapse of the PMF by 50 µM CCCP. (A–F). Visible in the left panels (Z-projection) are the results of averaging the fluorescence in the first 7.5 s into one image with a single frame acquisition time of 10 ms (A,C,E) and 30.5 ms (B,D,F). Signals observed in the cytosol are a combination of fast moving cytosolic (auto)fluorescence and out of focus signals originating from molecules within the cytoplasmic membrane on the axial axis. Second panel (Reconstruction), super-resolution reconstruction images showing signal detected at the cytoplasmic membrane. Colors indicate the frequency and accuracy of signal observed at the coordinate. Red indicating a low fit accuracy and/or frequency, whereas white signifies a high fit accuracy and/or frequency of fluorescence observed at that location. Third panel (Overlay), a merge of the super-resolution reconstruction with Z-projection to clarify the localization of SecA-Ypet. Fourth panel (Cross-section profile), a short axis cross section profile of the normalized fluorescence intensity distribution of each cell. Scale bar is 1 µm.