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. 2021 Jan 14;11:1433. doi: 10.1038/s41598-021-81081-2

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Cellular distribution profiles. (A) Cellular distribution profiles of the fluorescence for the membrane protein LacY-Ypet (Membrane, red solid line), cytosolic protein Ypet (Cytosolic, blue solid line) and SecA under native conditions (Black dashed line), treatment with 3 mM NaN₃ (Green dashed line) and treatment with 50 µM CCCP (Cyan dashed lines). (B) Z-projections of the membrane marker LacY-Ypet and cytosolic marker cytosolic Ypet. Plots are the result of averaging the fluorescence in the first 10 s into one image with a single frame acquisition time of 30 ms. Scale bar is 1 µm.