Table 2.
Diffusion data of cytosolic and membrane(-bound) proteins and probes.
| Protein/lipid dye | MM (kDa) | TM domains | Radius (nm) | Diffusion coefficient (µm2/s) | Fraction (%) | References |
|---|---|---|---|---|---|---|
| Cytosolic | ||||||
| eYFP | 26.7 | – | 3–4 | 7.08 ± 0.32 | 66 | |
| Crr-YFP | 45.0 | – | – | 2.03 ± 0.05 | 66 | |
| HtpG-YFP | 198.0 | – | – | 1.65 ± 0.07 | 66 | |
| SecA-Ypet | 130.3 | – | – | 2.09 ± 0.10 | 27 ± 1.6 | This study |
| + 3 mM NaN3 | 2.19 ± 0.03 | 21 ± 1.1 | This study | |||
| + 50 µM CCCP | 2.20 ± 0.04 | 48 ± 2.5 | This study | |||
| Membrane-bound | ||||||
| DiL-C12 | – | – | – | 0.365 ± 0.012 | 45 | |
| Bodipy FL-C12 | – | – | – | 1.502 ± 0.078 | 45 | |
| YedZ-eGFP | 24.1 | 6 | 1.3 | 0.188 ± 0.004 | 45 | |
| CybB-eGFP | 20.3 | 4 | 1.7 | 0.175 ± 0.008 | 45 | |
| GlpT-eGFP | 50.3 | 12 | 2.0 | 0.153 ± 0.003 | 45 | |
| Tar(1-397)-YFP | 142.3 | 4 | – | 0.217 ± 0.030 | 66 | |
| SecA-Ypet | 130.3 | – | – | 0.209 ± 0.016 | 51 ± 3.3 | This study |
| + 3 mM NaN3 | 0.190 ± 0.010 | 55 ± 1.5 | This study | |||
| + 50 µM CCCP | 0.160 ± 0.010 | 52 ± 2.5 | This study | |||
MM molecular mass. TM domains Transmembrane domains.