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. 2021 Jan 14;12(1):85. doi: 10.1038/s41419-020-03370-4

Fig. 3. Acquired resistance to PI3Kα inhibition in ESCC cells was associated with hyper-activation of mTORC1, MAPK, and c-Myc.

Fig. 3

A Parental and resistant cells were treated with CYH33 at the indicated concentrations for 1 h. Cell lysates were subjected to western blotting with indicated antibodies. B, C CYH33-resistant and parental cells were treated with 1 μM CYH33 or DMSO for 24 h and RNA was purified and subjected to RNA sequencing. GSEA enrichment plot of differentially expressed genes in the gene set of mTORC1 signaling pathway (HALLMARK_MTORC1_SIGNALING) (B) and MYC targets (HALLMARK_MYC_TARGETS_V1) (C) upon CYH33 treatment in CYH33-resistant cells and parental cells. D CYH33-resistant cells and parental cells were treated with CYH33 at indicated concentrations for 1 h. Cell lysates were subjected to western blotting with indicated antibodies. Data shown are from three replicates or three independent experiments.