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. 2021 Jan 14;11:1340. doi: 10.1038/s41598-020-78763-8

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Balanced Diet does not influence omega 6 PUFAs in plasma, but diet influences LA in WAT and BAT. (a) Plasma LA, ARA and DPA w3 does not differ between Balanced Diet (BD) AGS in all time points compared to Standard Rodent Chow (SRC) AGS (t-test, FDR corrected, fall n = 9, torpor n = 7–8, IBA n = 4). Seasonal comparison within the same diet found plasma LA increases in torpor compared to fall BD AGS while DPA w6 decreases in torpor and IBA compared to fall in both diets (post hoc Tukey, letters (a or b) signify difference between season within the same diet group). (b) WAT LA is increased in SRC AGS compared to BD AGS in fall, torpor and IBA, while ARA is increased in torpid SRC animals and DPA w6 is increased in BD AGS in torpor (*p < 0.05, **p < 0.001, t-test, FDR corrected, fall n = 11–12, torpor = 7–8, IBA = 4). Seasonal comparison within the same diet found WAT LA decreases in torpor compared to fall BD AGS (post hoc Tukey, letters (a or b) signify difference between season within the same diet group) c. BAT LA is significantly higher in SRC AGS compared to BD AGS in torpor and IBA (*p < 0.05, **p < 0.001, t-test, FDR corrected, torpor = 7–8, IBA = 4). Data shown are mean ± SEM. Black dots are individual data points.