Table 1.
Balanced Diet increases core body temperature in torpid AGS.
| (a) | Balanced diet ω 6:3 | Standard Rodent Chow |
|---|---|---|
| Core body temperature (°C) during Torpor | 3.3 (± 0.1)*, n = 9 | 2.8 (± 0.1), n = 8 |
| Core body temperature (°C) during Arousal | 35.6 (± 0.1), n = 9 | 35.5 (± 0.1), n = 8 |
| First Torpor bout length (h) | 136.2 (± 20.4) n = 9 | 172.1 (± 29.0) n = 8 |
| Second Torpor bout length (h) | 237.7 (± 14.5) n = 9 | 237.4 (± 24.8) n = 8 |
| Third Torpor bout length (h) | 315.8 (± 18.4) n = 9 | 303.6 (± 28.6) n = 8 |
| Fourth Torpor bout length (h) | 351.7 (± 15.6) n = 9 | 370.6 (± 26.7) n = 7 |
| Fifth Torpor bout length (h) | 375.8 (± 19.5) n = 8 | 388.3 (± 23.6) n = 7 |
| (b) | Balanced diet ω 6:3 | Standard Rodent Chow |
|---|---|---|
| Percent total fatty acid | ||
| Palmitic acid (16:0) | 13.99 | 12.49 |
| Palmitoleic acid (16:1ω7) | 1.19 | 0.24 |
| Stearic acid (18:0) | 4.89 | 3.44 |
| Oleic acid (18:1ω9) | 24.67 | 20.96 |
| Linoleic acid (18:2ω6) | 29.58 | 50.50 |
| Alpha linolenic acid (18:3ω3) | 19.81 | 10.04 |
| Arachidonic acid (20:4ω6) | 0.20 | 0.02 |
| EPA (20:5ω3) | 0.69 | 0.04 |
| DHA (22:6ω3) | 0.96 | 0.11 |
| Tot.ω3 | 21.81 | 10.24 |
| Tot.ω6 | 30.09 | 50.67 |
| Tot.ω9 | 24.77 | 21.01 |
| Ratio ω6/ω3 | 1.38 | 4.95 |
| Concentration (mg/100 g chow) | ||
| Total fat | 7326.34 | 7484.87 |
| Total PUFA | 3620.8 | 4342.3 |
(a) Hibernating core body temperature is significantly higher in Balanced Diet fed AGS (*p = 0.038, t-test, n = 8–9), but diet did not influence arousal core body temperature or torpor bout length. Data shown are mean ± SEM. (b) Fatty acid composition of Balanced Diet (Lab Diet, 9GU5) and Standard Rodent Chow (Mazuri, #5663). Data shown are percent of total fatty acid, except total fat and total PUFA are mg/100 g chow. Full fatty acid composition is listed in Supplemental Table 7.