White fat, brown fat, and estrogen-associated gene expression in inguinal fat pads from the ERα−/− mice after EPO treatment for 4 weeks on the HFD. (A–C) Expression of white adipose tissue-related genes (A), brown fat-associated genes (B), and estrogen-related genes (C) in inguinal fat pads from the female WT and ERα−/− mice at 4 months (n = 5–6/group). (D) Protein expression of UCP1, Cidea, PRDM16, pERK/ERK, pAKT/AKT, and pJNK/JNK in inguinal fat pads from the female WT and ERα−/− mice at 4 months (n = 3–4/group, ∗p < 0.05, and ∗∗p < 0.01).