Figure 6.

Adipocyte sizes and gonadal fat pad gene expression in the mice with targeted deletion of ERα in adipocyte tissues. (A–C) Adipocyte size distribution from female gonadal fat pad sections of the WT and ERα^adipoKO mice at 4 months on the HFD for 12 weeks with 2 weeks of EPO treatment. Representative hematoxylin and eosin staining (A; scale bar: 50 μm), average cell size (B), and cell size distribution (C). Symbols indicate the WT PBS (filled circle, solid line) and EPO (open square, dotted line)-treated mice and ERα−/− PBS (red filled triangle, solid line) and EPO (red open inverted triangle, dotted line)-treated mice. (D–E) Expression of proinflammatory genes (D; n = 6–9/group) and iNOS protein levels (E; n = 3–4/group) in inguinal fat pads from the WT and ERα^adipoKO females at 4 months on the HFD for 12 weeks with EPO or PBS treatment for the last 2 weeks. ∗p < 0.05 and ∗∗p < 0.01. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)