Fat pad gene expression and signaling with EPO treatment in the mice with targeted deletion of ERα in adipocyte tissues. (A–B) Expression of white fat-associated genes in inguinal fat pads from the female WT and ERαadipoKO mice (n = 5–6/group) at 4 months on the HFD for 12 weeks with 2 weeks EPO treatment (A) and in 3T3L1 cells (B) treated with EPO, E2, or insulin (n = 4/group). In (B), ∗ means PBS vs treatment group without insulin treatment and † means PBS vs treatment group with insulin treatment. (C–D) Expression of brown fat-associated genes (C; n = 5–6/group) and protein levels of UCP1 and pERK/ERK (D; n = 3–4/group) in inguinal fat pads from the female WT and ERαadipoKO mice at 4 months on the HFD for 12 weeks with 2 weeks EPO treatment. ∗, †p < 0.05, ∗∗, ††p < 0.01.