Figure 3.

E. coli and S. enterica, but not P. aeruginosa LPS signal partially via the endosome in polarized macrophages resulting in altered inflammatory cytokine production. Differentiated M1 THP-1 macrophages were stimulated with LPS from P. aeruginosa, E. coli or S. enterica with or without pre-incubation with 100 µM Hydroxy-Chloroquine. Induction of (A) LPS induced P65 phosphorylation measured after 30 min with/without Clq (Hydroxychloroquine). (B–E) IP-10, TNFα, IL-6 and IL-8 production was measured in conditioned supernatants. Data is expressed as the mean ± SEM of 3 independent differentiations of THP-1 derived macrophages of passages 5–8. Statistical analysis performed on qPCR data was a one-way ANOVA with Tukeys posthoc test, **P < 0.01, ****P < 0.0001.