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. 2021 Jan 14;11:1372. doi: 10.1038/s41598-020-80621-6

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© The Author(s) 2021, corrected publication 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of cholangiocyte activation and inhibition on hepatocyte proliferation, apoptosis and fibrosis. 3-dpf kras + and WT zebrafish larvae were treated with either TCA or JTE-013 along with 20 µg/mL Dox till 8 dpf. Samples were collected for immunohistochemistry. kras+ and WT liver sections were incubated with primary antibodies for PCNA, Caspase 3a, Collagen or Laminin and then stained with Alexa Fluor 546 conjugated secondary antibody. All liver sections were counter-stained with DAPI. (A,C, E,G) Representative images of staining for PCNA (A), Caspase 3a (C), Collagen (E) and Laminin (G) of kras+ and WT zebrafish larvae in different groups. (B,D, F,H) Quantification of staining signals. For PCNA and Caspase 3a staining, number of stained cells were quantified. For Collagen and Laminin stainings, stained areas were quantified. N = 10 each group. Scale bar: 20 μm. Statistical significance: *P˂0.05.