Figure 3.

Vagal nerve stimulation (VNS) decelerates the development of endometriosis in mouse. (A) Kinetics of the mean bodyweight in control and VNS groups. No significance was found between two groups at any time point evaluated. (B) Kinetics of the mean hotplate latency, tested at the indicated times in the two groups. Statistically significant difference in latency between the two groups is found at the end of the experiment, 2 weeks after the induction of endometriosis. (C) Boxplot summarizing the lesion weight data between control and VNS mice. Representative immunostaining results for E-cadherin (D) and vimentin (E) in endometriotic lesions from control and VNS mice (left panel), along the boxplot summarizing the staining data (right panel). (F) Representative images of Masson trichrome staining in endometriotic lesions in control and VNS groups (left panel), along with the boxplot summarizing the staining data (right panel). n = 10 in each group. The asterisk indicates the statistical significance level, based on linear regression using group indicator: NS: p > 0.05; *p < 0.05; **p < 0.01. The R² values for E-cadherin and vimentin were 0.51 and 0.27, respectively. For the exent of fibrosis, R² = 0.10.