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. 2021 Jan 14;11:1325. doi: 10.1038/s41598-020-79384-x

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Expression of lamin A/C and lamin B receptor during porcine in vitro adipogenesis. (a) Relative transcript levels of the LMNA gene over seven days of differentiation. Different capital letters refer to significant differences between days (P < 0.05); error bars show SD. (b) Comparison of immunofluorescent signal intensities of lamin A/C normalized by DAPI fluorescence intensity signal on days 0 and 7 of in vitro adipogenesis; error bars show SD. Statistically significant differences (P < 0.05) are marked with asterisks. The number of nuclei analyzed was n = 169. (c) Relative transcript levels of LBR gene on subsequent days of adipogenic differentiation. Different capital letters refer to significant differences between analyzed days (P < 0.05); error bars show SD. (d) Comparison of immunofluorescent signal intensities of LBR normalized by DAPI fluorescence intensity on days 0 and 7 of in vitro adipogenesis; error bars shows SD. Statistically significant differences (P < 0.05) are marked with asterisks. The number of nuclei analyzed was n = 280. (e) Visualization of lamin A/C (red) or LBR (green) by indirect immunofluorescent staining in undifferentiated MSC cells (day 0) and adipocytes (day 7). Lipid droplets were stained using BODIPY (green) or visualized in brightfield and nuclei were counterstained with DAPI (blue). Scale bar: 10 µm.