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. 2021 Jan 14;11:1325. doi: 10.1038/s41598-020-79384-x

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Spatial distribution of selected histone marks associated with euchromatin. (a) Distribution of H4K8ac, H3K4me3, and H3K9ac in a 3D nuclear space on days 0 and 7 of in vitro adipogenesis. The histone marks were visualized by indirect immunofluorescence (green). Nuclei were counterstained with DAPI (blue). Scale bar: 10 µm. (b) The graphs show the spatial distribution of immunofluorescent signals in 3D nuclear space. Statistically significant differences (P < 0.05) are marked with asterisks. Number of nuclei analyzed: H4K8ac: n = 551, H3K4me3: n = 594, H3K9ac: n = 653. Statistical data are shown in Supplementary Table 2.