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. 2021 Jan 14;11:1314. doi: 10.1038/s41598-020-80263-8

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Radiographic and immunohistochemical features of HBs. (a–c) Axial, sagittal and coronal contrast enhanced T1-weighted MRI of a solid HB showing well-circumscribed solid mass in the right cerebellar hemisphere. (d–f) Axial, sagittal and coronal contrast enhanced T1-weighted MRI of a cystic HB showing a non-enhanced cystic mass in the right cerebellar hemisphere with an enhancing mural nodule. The enhanced tumor nodule contained tumor cells, and surrounding cyst consisted of plasma ultrafiltrates. (g) HE staining demonstrated vascular and stromal cells in cystic HBs, ×100. Immunohistochemical staining of cystic HBs revealed tumor cells staining positive for carbonic anhydrase IX (CAIX) (h); endothelial cell marker (CD34) (i); 2-phospho-D-glycerate hydrolase (NSE) (j); SOX9 (k) and negative for phosphoenolpyruvate Carboxykinase (PCK) (l), ×400.