Skip to main content
. 2021 Jan 14;41(1):BSR20203130. doi: 10.1042/BSR20203130

Figure 1. Circ_0061012 is highly expressed in the lesional skin of psoriasis patients and IL-22-induced HaCaT cells.

Figure 1

(A) The expression of circ_0061012 was examined in the lesional skin of psoriasis patients (n=27) and healthy skin of normal volunteers (n=27) by qRT-PCR. (B) The level of circ_0061012 was determined in HaCaT cells induced by IL-22 of 50, 100 or 200 ng/ml for 24 h via qRT-PCR. (C) The proportion of circ_0061012 distribution in cytoplasmic fraction or nuclear fraction was analyzed. U6 and GAPDH were utilized as the nuclear marker and cytoplasmic marker, respectively. (D) Exonuclease RNase R was utilized to assess if circ_0061012 was a circular transcript. RNA samples isolated from HaCaT cells were digested with RNase R or not, and the levels of circ_0061012 and its linear counterpart were measured by qRT-PCR. The experiments were independently repeated for three times with at least three technical repetitions. *P<0.05, ****P<0.0001.