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. 2021 Jan 14;41(1):BSR20203130. doi: 10.1042/BSR20203130

Figure 6. MiR-194-5p suppresses IL-22-induced progression of psoriasis partly through targeting GAB1 in HaCaT cells.

Figure 6

(A–E) HaCaT cells transfected with miR-NC, miR-194-5p, miR-194-5p + pcDNA or miR-194-5p + GAB1 were treated with 100 ng/ml IL-22 for 24 h. (A) Western blot assay was utilized to measure the protein level of GAB1 in HaCaT cells. (B) MTT assay was used to evaluate cell viability of HaCaT cells at specific time points, and cell proliferation curve was plotted to evaluate cell proliferation capacity. (C,D) Transwell migration and invasion assays were used to measure the abilities of migration and invasion of HaCaT cells. The numbers of migrated and invaded HaCaT cells (stained by Crystal Violet) in five random fields were counted. Scale bar = 100 μm. (E) Western blot assay was used to measure the levels of Ki67 and MMP9 in HaCaT cells. The experiments were independently repeated for three times with at least three technical repetitions. *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001.