FIGURE 4.

LDHA was a transcriptional target of FOXO4 in GC. (A) By using the JASPAR CORE database, we identified a series of putative binding sites in LDHA promoter for FOXO4 with calculated z‐score from 11.037 to 2.641. (B) Luciferase assays demonstrated that FOXO4 significantly decreased the luciferase activity of LDHA in both AGS and MGC803 cells in a dose‐dependent manner. (C) Schematic of ChIP analysis in LDHA promoter. (D) Chromatin immunoprecipitation analysis demonstrated that FOXO4 was directly bound to the region from ‐501 to ‐1013 bp of the LDHA promoter (sites 2#)