Figure 2.

MAKR2 Mediates PIN2-GFP Dynamic Accumulation during Gravitropism

(A) Confocal pictures of the MAKR2prom::MAKR2-tdYFP line showing the MAKR2-tdYFP localization and expression pattern at the root tip. Left: yellow fluorescent protein (YFP) channel; right: overlay between YFP channel (yellow) and membranes counterstained by FM4-64 (red).

(B) Confocal pictures of the complemented ROP6prom::mCitrine-ROP6/rop6-2 line showing the mCit-ROP6 localization and expression pattern at the root tip.

(C) Kinetics of root gravitropic bending after reorienting seedlings at a 135° angle. See Figure S1F for a statistical comparison. A linear model was fitted on measurements from wild-type plants and the different mutants using lm() function from stats package available in R software (https://www.r-project.org/). This model estimates a weight for each variable (wild-type and mutant plants) and the associated probability that such weight is different from zero based on a t test. The probability derived from the t test is the p value in this comparison and significant differences were considered when p < 0.05.

(D) Quantification of PIN2-GFP in the upper (blue diamonds) and lower (black squares) part of the root in the PIN2prom::PIN2-GFP, PIN2prom::PIN2-GFP;MAKR2-Ox1, and PIN2prom::PIN2-GFP;amiMAKR2.1 lines. Each graph shows the response in a single individual root (see also Videos S1 and S2). In each case, fluorescence intensities were normalized with respect to the initial fluorescence value (time 0 min).

Scale bars represent 30 μm.

See also Figures S1 and S2 and Videos S1 and S2.