REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
b-III-tubulin	Biolegend	Cat#801201; RRID: AB_2313773
b-III-tubulin	Biolegend	Cat#802001; RRID: AB_2564645
Ki67	BD Bioscience	Cat#550609; RRID: AB_393778
APP C-terminal fragment	Biolegend	Cat#802803; RRID: AB_2715853
Aβ (6E10)	Biolegend	Cat#803014; RRID: AB_2728527
PSEN1	Millipore	Cat#MAB5232; RRID: AB_95175
Notch ICD (Val 1744)	Cell Signaling Technologies	Cat#4147; RRID: AB_2153348
β Actin	Sigma	Cat#1978; RRID: AB_476692
FOXG1	Abcam	Cat#ab18259; RRID: AB_732415
NESTIN	Santa Cruz	Cat#sc-23927; RRID:AB_627994
PAX6	Biolegend	Cat#901301; RRID:AB_2565003
Chemicals, Peptides, and Recombinant Proteins
Dispase	ThermoFisher	17105-041
Accutase	ThermoFisher	A11105-01
SB431542	R&D Systems	1614/10
Dorsomorphin	R&D Systems	3093/10
N2 Supplement	ThermoFisher	17502-048
B27 Supplement	ThermoFisher	17504-044
POWER Sybr green	ThermoFisher	4368702
Trizol	ThermoFisher	15596-026
Essential 8 medium	ThermoFisher	A1517001
Laminin	Sigma	L2020
Matrigel	Corning	356255
DAPT (γ-secretase inhibitor N-[N-(3, 5-Difluorophenacetyl)-L-alanyl]-S-phenylglycine t-butyl ester)	Tocris	2634
LY2886721	Cambridge Bioscience	2299-5
Superscript IV	ThermoFisher	18091050
Geltrex	ThermoFisher	A1413302
Critical Commercial Assays
Human (6E10) Aβ42 Ultra-Sensitive Kit	MesoScale Discovery	K151FUE
Experimental Models: Cell Lines
Ctrl 1 - Human iPSC	Dr Tilo Kunath; Arber et al., 2020	N/A
Ctrl 2 - Human iPSC	Coriel cell repository	ND41886
Ctrl 3 - Human iPSC	Sigma	RBi001-a
Ctrl 4 - Human iPSC	Sigma	SIGi1001-a-1
Shef6 - Human ESC	UK Stem Cell Bank	N/A
APP V717I patient 1 (2 clones) - Human iPSC	Stembancc	N/A
APP V717I patient 2 - Human iPSC	Arber et al., 2020	N/A
PSEN1 int4del (2 clones) - Human iPSC	Stembancc	N/A
PSEN1 Y115H - Human iPSC	Arber et al., 2020	N/A
PSEN1 M139V - Human iPSC	Stembancc	N/A
PSEN1 M146I - Human iPSC	Stembancc	N/A
PSEN1 R278I (2 clones) - Human iPSC	Arber et al., 2020	N/A
Oligonucleotides
RPL18a Fwd	CCCACAACATGTACCGGGAA	Arber et al., 2020
RPL18a Rev	TCTTGGAGTCGTGGAACTGC	Arber et al., 2020
FABP7 Fwd	CAAGAACACGGAGATTAG	This study
FABP7 Rev	GCTAACAACAGACTTACA	This study
TUBB3 Fwd	CATGGACAGTGTCCGCTCAG	Arber et al., 2020
TUBB3 Rev	CAGGCAGTCGCAGTTTTCAC	Arber et al., 2020
Other
Expression data from human fetal tissue aged 15 – 21 weeks post conception	Miller et al., 2014	Miller et al., 2014
Single Cell RNaseq “dataset A” - dentate gyri from P12-P35 mice	Hochgerner et al., 2018	GEO: GSE95315