Figure EV1. Oligo(ADP‐ribose) or short PAR chains are synthesized on H2AX following DNA damage.

1. Smeared band of ADP‐ribosylated H2AX was observed with longer exposure. 293T cells were treated with H₂O₂ (2 mM in PBS, 5 min), MMS (1 mM in medium, 30 min), or mock (PBS, 30 min). ADP‐ribosylated proteins were immunoprecipitated with anti‐ADPR antibody and examined by Western blotting using anti‐H2AX antibody.
2. 293T cells were treated with H₂O₂ (2 mM in PBS, 5 min), MMS (1 mM in medium, 30 min), or mock (PBS, 30 min). PARylated proteins were immunoprecipitated with anti‐PAR antibody. PARylated‐H2AX was examined by Western blotting using anti‐H2AX antibody.

Source data are available online for this figure.