Figure EV3. Hyper‐induction of IFNγ and granzyme B in Peli1‐deficient CD8 T cells is dependent on mTORC1.

• A, B
  Flow cytometry analysis of intracellular IFNγ (A) and granzyme B (B) in OT‐I naïve T cells that were either not treated (NT) or stimulated for 16 h with plate‐coated anti‐CD3 (1 μg/ml) and anti‐CD28 (1 μg/ml) in the presence of DMSO or rapamycin (10 nM). Data are repeated with 4 independent experiments, and the results were presented as a representative plot (left) and summary graph of mean ± SEM values based on multiple mice (right). P values were determined by two‐tailed unpaired Student’s t‐test. *P < 0.05; **P < 0.01.