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. 2020 Nov 20;40(2):e104532. doi: 10.15252/embj.2020104532

Figure EV4. Peli1 does not regulate AMPK activation or mediate ubiquitination of LKB1 and AMPK.

Figure EV4

  • A
    Immunoblot analysis of the phosphorylated (P‐) and total proteins in whole‐cell lysates of CD8 T cells stimulated for the indicated time points.
  • B–D
    Ubiquitination of LKB1 (B), AMPKα1 (C), and AMPKα2 (D) in 293 cells transfected with the indicated expression vectors. Ubiquitinated proteins were isolated by immunoprecipitation and detected by immunoblot (upper), and the protein expression levels in input cell lysates was analyzed by immunoblot (lower).