Figure 5. Peli1 regulates mTORC1 activation by growth factors in T cells and MEF cells.

• A, B
  Immunoblot analysis of the indicated phosphorylated (p‐) and total proteins in whole‐cell lysates of wild‐type (WT) or Peli1‐KO (KO) OT‐I CD8 T cells that were activated for 16 h with plate‐coated anti‐CD3 (1 μg/ml) plus anti‐CD28, starved for 6 h in serum‐free RPMI medium, and then treated for indicated time periods with FBS (10%) (A) or insulin (B).
• C–E
  Immunoblot analysis of the indicated phosphorylated (p‐) and total proteins in whole‐cell lysates of wild‐type (WT) or Peli1‐KO (KO) MEFs that were starved for 16 h in serum‐free DMEM medium and then treated for the indicated time periods with FBS (C), insulin (D), and EGF (E).

Source data are available online for this figure.