Figure EV5. Sites of Peli1‐mediated TSC1 ubiquitination.

1. Immunoprecipitation and immunoblot analysis of TSC1 ubiquitination in 293 cells transfected with (+) or without (–) the indicated expression vectors.
2. Summary of ubiquitination sites of TSC1 detected by mass spectrometry in cells coexpressing TSC1 and Peli1. TSC1 was isolated by anti‐Flag immunoprecipitation from 293T cells transiently transfected as indicated in a. Samples from lanes 2 and 3 were analyzed by mass spectrometry.
3. Sequence alignment of TSC1 ubiquitination sites.