Figure EV4. Rabl2^D73G does not repress ciliogenesis and ciliary exit of BBSome and SMO (related to Fig 6).

1. Rabl2^D73G did not affect ciliogenesis efficiency. RPE1 cells that were either uninfected (control) or infected with adenovirus to express GFP‐tagged Rabl2^Q80L or Rabl2^D73G were serum‐starved for 48 h. The white arrow denotes cilium. At least 100 cells were scored in each experiment and condition.
2. Rabl2^D73G did not cause ciliary BBSome accumulation. Rabl2^Q80L served as positive control. The white arrows indicate positions of cilia. At least 105 cells were scored in each experiment and condition.
3. Rabl2^D73G did not alter ciliary translocation of SMO. RPE1 cells expressing GFP‐Rabl2^D73G were serum‐starved for 24 h and treated with DMSO (mock) or SAG for an additional 24 h prior to immunostaining. The white arrows indicate positions of cilia. At least 100 cells were scored in each experiment and condition.

Data information: Quantification results are presented as mean ± s.d. Student's t‐test: ns, no significance; ***P < 0.001.