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. 2020 Dec 4;40(2):e106123. doi: 10.15252/embj.2020106123

Figure 2. Soft tumor cells have the ability to form tumors in mice.

Figure 2

  1. Soft or stiff 4T1 or MCF‐7 cells were isolated by the microfluidic chip, and then seeded (500 cells) in 90 Pa soft 3D fibrin gel for 3 days. The percentage of colonies formed was calculated and the colony size was recorded. Scale bar, 100 μm.
  2. The soft or stiff 4T1 or MCF‐7 cells (100 cells) were injected into the mammary fat pads of NSG or BALB/c mice. Twelve weeks later, the tumor formation was recorded. n = 8–10.
  3. The same as (B), except that different number of 4T1 cells were injected into the BALB/c mice. n = 10.
  4. The tumor‐forming capacity from primary xenografts (F1) and tumors passaged into secondary (F2) and tertiary (F3) recipients induced by injecting 100 soft 4T1 or B16 cells into the BALB/c or C57BL/6 mice. n = 10.
  5. Stiff or soft B16 or MP‐1 cells (100 or 10 cells) were injected into the NSG mice by tail veil. n = 8–12.
  6. NSG mice were intravenously injected with 100 soft or stiff B16 or MP‐1 cells. Eight weeks later, the lung metastasis was analyzed by H&E staining. The metastasis index was defined as the percentage of total metastatic nodule area to the total lung area based on the calculation from 10 slides. Scale bar, 0.5 mm. n = 3 (for B16) or 5 (for MP‐1) mice with metastatic tumor.

Data information: Two‐tailed Paired Student’s t‐test (A and F). The data represent mean ± SD. n = 3 independent experiments in (A).