Figure EV3. Internalization of 5xFAD hAPP and p75NTR in wild‐type mouse hippocampal neurons.

1. Quantification of cell surface hAPP in wild type and p75NTR mutant neurons after 6E10 antibody feeding on ice followed by fixation. Values were normalized to levels in wild‐type neurons and are expressed as percentage ± SEM. N = 3 independent experiments.
2. Internalization of 5xFAD hAPP in wild‐type mouse hippocampal neurons. Live neuron cultures were fed with anti‐human APP antibodies (6E10) on ice, washed, and then placed at 37°C for different periods of time to allow internalization. The reaction was stopped by a quick acid wash followed by fixation. Total staining (100%) was determined by direct fixation after antibody feeding. Baseline (t = 0 min) was obtained by acid wash directly after antibody feeding. Counterstaining for p75NTR (antibody GT15057, see Table S1) and DAPI is also shown. Scale bar, 10 μm.
3. Quantification of cell surface p75NTR in wild type and mutant neurons after antibody feeding on ice followed by fixation. Values were normalized to levels in wild‐type neurons and are expressed as percentage ± SEM. N = 3 independent experiments.
4. Internalization of p75NTR in wild‐type mouse hippocampal neurons. Live neuron cultures were fed with anti‐mouse p75NTR antibodies on ice, washed, and then placed at 37°C for different periods of time to allow internalization. Counterstaining for MAP2 and DAPI is also shown. Scale bar, 10 μm.